流行性出血热冻干致敏人O型血球的研制及其应用

本文应用致敏的人O型血球研究反向间接血凝(RPHA)和反向间接血凝抑制(RPHI)方法用以检测流行性出血热抗原抗体,并试验成功用pH9.0硼酸盐水制备灭活鼠脑病毒液作为抗原。为抗原制备提供了一种简便的方法。以上RPHA法用于检测组织培养内病毒与用荧光法检测细胞内病毒抗原法结果一致,用RPHI检测病人血清抗体效价,特异性高,敏感性与IFA相同。该致敏血球和抗原是冻干制品,稳定性好、使用方便,是一种代替荧光检测病毒抗原和抗体的良好制品。

Use of AntibOdy Sensitized Human O Red Blood Cells for Reverse Passive Hemagglutination Test(RPHA) and RPHA Inhibition Test(RPHI) for Detection of Antigen and Antibody

We have studied RPHA and RPHI test using anti-EHFV antibody sensitized human red blood cell for detecting EHF antigen and antibody. The antigen used in RPHI was successfully prepared with infected mouse brains extracted by pH 9.0 borate buffered saline(BBS), providing a method of simplicity for preparing antigen. This RPHA test can be used for detection on antigen content of EHF vaccine instead of ELISA, and for detection of EHF virus in tissue culture fluid instead of using IF technique for detecting EHF antigen in cells. The sensitivity and specificity of RPHI method for diagnosis of EHF patients were proved to be identical to the IF method,but the RPHI was much simpler and easier to carry out than IF.