Plant regeneration from cotyledons of niger |
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Authors: | A Sarvesh T P Reddy P B Kavi Kishor |
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Institution: | (1) Department of Genetics, Osmania University, 500 007 Hyderabad, India |
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Abstract: | Callus initiation from seedling explants of niger (Guizotia abyssinica Cass) cv. Ootacamund was found to be better on LS medium containing kinetin (1.4 M) plus 2,4-dichlorophenoxyacetic acid (9 M) than its analogues. Embryoids were induced directly from cotyledons on LS medium supplemented with 2,4,5-trichlorophenoxyacetic acid and 2,4,5-trichlorophenoxypropionic acid. When cotyledon-derived callus was subcultured onto medium with 10.7 M naphthalene-acetic acid and 2.3 M kinetin, embryogenesis was observed. Multiple shoots were obtained from cotyledonary explants in presence of MS medium containing 4.4 M benzyladenine and 11.4 M indoleacetic acid. Regenerated plants that were transferred to pots and grown to maturity were morphologically normal and fertile.Abbreviations NAA
naphthaleneacetic acid
- IAA
indoleacetic acid
- BA
benzyladenine
- GA3
gibberellic acid
- 2,4-D
2,4-dichlorophenoxyacetic acid
- 2,4,5-T
2,4,5-trichlorophenoxypropionic acid
- 2,4,5-TP
2,4,5-trichlorophenoxypropionic acid
- ABA
abscisic acid |
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Keywords: | callus Guizotia abyssinica Cass |
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