Effect of Bcl-2 Expression on Hybridoma Cell Growth in Serum-Supplemented, Protein-Free and Diluted Media |
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Authors: | D Fassnacht S Rössing F Franěk M Al-Rubeai R Pörtner |
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Institution: | 1.Technische Universität Hamburg-Harburg, Bioprozeß- und Bioverfahrenstechnik, Denickestrasse 15, D-21071 Hamburg, Germany ;2.Czech Academy of Science, Institute of Molecular Genetics, CZ-14220 Praha 4, Czech Republic ;3.Center for Biochemical Engineering, School of Chemical Engineering, University of Birmingham, Edgbaston, Birmingham, B15 2TT U.K |
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Abstract: | Two transfected hybridoma cell lines TB/C3-bcl2 (overexpressing the Bcl-2 protein) and TB/C3-pEF (control cell line), were
compared in batch suspension cultures using a medium supplemented either with horse serum or with a protein-free, iron-rich
supplement. The membrane intact index (percentage of cells with intact membranes determined by trypan blue staining) of the
TB/C3-bcl2 cell line decreased much slower than that of the control cell line during the dying phase of the cultures. No significant
difference in antibody, lactate and ammonia production as well as glucose and glutamine consumption was noted in the exponential
phase of the experiments. Both cell lines were also compared in batch experiments using media diluted with saline to further
investigate the effect of Bcl-2 under sub-optimal conditions. The Bcl-2 overexpressing cell line again exhibited a higher
membrane intact index at increasing dilution steps.
This revised version was published online in June 2006 with corrections to the Cover Date. |
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Keywords: | apoptosis Bcl-2 diluted medium hybridoma protein-free medium |
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