Involvement of miR-21 in resistance to daunorubicin by regulating PTEN expression in the leukaemia K562 cell line |
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| Authors: | Bai Haitao Xu Rang Cao Zhongwei Wei Daolin Wang Chun |
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| Institution: | aDepartment of haematology, Shanghai Jiaotong University Affiliated Shanghai First People’s Hospital, Shanghai, China;bScience and Research Center, Shanghai Jiaotong University Affiliated Shanghai Xinhua Hospital, Shanghai, China;cDepartment of Gastroenterology, Shanghai Jiaotong University Affiliated Shanghai First People’s Hospital, Shanghai, China |
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| Abstract: | Recent studies have shown microRNA-21 (miR-21) is overexpressed in several types of cancer and contributes to tumor resistance to chemotherapy. In this study, we investigated whether miR-21 mediated resistance of the leukaemia cell line K562 to the chemotherapeutic agent daunorubicin (DNR). miR-21 expression was upregulated in the DNR resistant cell line K562/DNR compared to its parental line K562. Stable transfection of miR-21 induced drug resistance in K562, while suppression of miR-21 in K562/DNR led to enhanced DNR cytotoxicity. Additional experiments indicate that the mechanism of miR-21 drug resistance involves the PI3K/Akt pathway and changes following PTEN protein expression. This study provides a novel mechanism for understanding leukaemia drug resistance. |
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| Keywords: | Leukaemia miR-21 Daunorubicin Drug resistance PTEN PI3K/Akt |
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