灰树花多糖对乳腺癌细胞MCF-7的凋亡作用

采用MTT法测定不同给药浓度的灰树花多糖(PGF) (1、10、20、50、100和200 μg/mL)在24、48和72 h对乳腺癌细胞(MCF-7)增殖的抑制率，并采用Hoechst染色与流式细胞技术观察20、50和100 μg/mL PGF给药24 h后MCF-7的凋亡情况，同时采用Western blotting对20、50、100 μg/mL PGF给药24 h后MCF-7细胞中Bax、Bcl-2、Pro-Caspase-3以及Cleaved Caspase-3的蛋白表达水平进行检测。研究发现PGF给药24、48和72 h后对MCF-7的增殖均有显著的抑制作用。随着PGF给药浓度增加，MCF-7细胞核裂解增多，细胞凋亡数量增多。PGF 20、50和100 μg/mL给药对MCF-7细胞Bax、Bcl-2、Pro-Caspase-3以及Cleaved Caspase-3的蛋白表达水平可见显著性差异。

Breast cancer cell MCF-7 apoptosis induced by polysaccharides of Grifola frondosa

The inhibition rate of proliferation of breast cancer cell MCF-7 treated with different concentration of polysaccharides of Grifola frondosa (PGF) for 24, 48 and 72 h was investigated. The MCF-7 cell apoptosis after PGF treatment under different concentration for 24 h was visualized by Hoechst staining and flow cytometry. The expression of apoptotic factors such as Bax, Bcl-2, Pro-Caspase-3 and cleaved Caspase-3 were detected by Western blotting. Cell viability analysis showed that PGF could inhibit proliferation of MCF-7 cells significantly and the inhibition rate was dependent on dose and treatment time of PGF. Apoptotic cells were increased with the increase of PGF concentration. Treatments of PGF under concentration of 20, 50 and 100 μg/mL significantly regulated the expression of Bax, Bcl-2, Pro-Caspase-3 and Cleaved Caspase-3 in MCF-7 cell.