Analysis of Assembly and Budding of Lujo Virus |
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| Authors: | Shuzo Urata Jacqueline Weyer Nadia Storm Yukiko Miyazaki Petrus Jansen van Vuren Janusz Tadeusz Paweska Jiro Yasuda |
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| Affiliation: | aDepartment of Emerging Infectious Diseases, Institute of Tropical Medicine (NEKKEN), Nagasaki University, Nagasaki, Japan;bThe Graduate School of Biomedical Science, Nagasaki University, Nagasaki, Japan;cCenter for Emerging and Zoonotic Diseases, National Institute for Communicable Diseases (NICD), Division of the National Health Laboratory Service, Sandringham, South Africa |
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| Abstract: | The recently identified arenavirus Lujo virus (LUJV) causes fatal hemorrhagic fever in humans. We analyzed its mechanism of viral release driven by matrix protein Z and the cell surface glycoprotein precursor GPC. The L domains in Z are required for efficient virus-like particle release, but Tsg101, ALIX/AIP1, and Vps4A/B are unnecessary for budding. LUJV GPC is cleaved by site 1 protease (S1P) at the RKLM motif, and treatment with the S1P inhibitor PF-429242 reduced LUJV production. |
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