Effects of 5-hydroxylysine on acetylene reduction and NH4+-assimilation in the cyanobacterium Anabaena cylindrica. |
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Authors: | J K Ladha P Rowell W D Stewart |
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Institution: | Department of Biological Sciences, University of Dundee, Dundee, Scotland DD1 4HN, U.K. |
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Abstract: | 5-hydroxylysine, an analogue of glutamate and lysine, causes production by N2-fixing A. cylindrica; it also reversibly inhibits GS activity in vitro but has no effect on alanine dehydrogenase or GOGAT. On adding 5-hydroxylysine intracellular pools of glutamine, glutamate and aspartate decrease; those of alanine and serine increase. 5-hydroxylysine alleviates the inhibitory effect of on heterocyst production and C2H2 reduction and in cultures results in heterocyst synthesis and in C2H2 reduction. The data suggest that the GS-GOGAT pathway is the sole route of importance in primary assimilation in A. cylindrica, that alone does not inhibit nitrogenase and heterocyst production, and that GS and/or a product is involved in regulating the production of both. |
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