普通枇杷、大渡河枇杷和栎叶枇杷遗传关系研究——基于RAPD和AFLP分析

利用筛选得到的24个随机引物和5对AFLP引物组合对普通枇杷、大渡河枇杷及栎叶枇杷的遗传关系进行RAPD及AFLP分析,结果表明,普通枇杷与栎叶枇杷的相似性系数最小(0.3864和0.5364),而大渡河枇杷介于普通枇杷和栎叶枇杷之间,偏向于栎叶枇杷。分别以普通枇杷、大渡河枇杷和栎叶枇杷作为待定杂种,计算了三者谱带的叠加性,结果表明,无论是RAPD分子标记,还是AFLP分子标记,以大渡河枇杷作待定杂种获得最高叠加性,支持大渡河枇杷可能是普通枇杷与栎叶枇杷的杂交种的结论。

The Genetic Relationship among Common Loquat, Daduhe Loquat and Oakleaf Loquat, According to RAPD and AFLP Analysis

Twenty-four random primers and 5 AFLP primer-combinations were used to assess the genetic relationship among common loquat, Daduhe loquat and Oakleaf loquat by RAPD and AFLP analysis.The results showed that genetic relationship between common loquat and Oakleaf loquat was the least, Daduhe loquat was located in between common loquat and Oakleaf loquat, and more close to Oakleaf loquat.On the other hand, we calculated the band's additivity when common loquat, Daduhe loquat and Oakleaf loquat were viewed as putative hybrid, the outcome suggested that in RAPD or AFLP analysis, the band's additivity was the highest when using Daduhe loquat as putative hybrid and the additivity was decreased when common loquat or Oakleaf loquat were examined as the hybrid.The results above suggested that Daduhe loquat is perhaps a hybrid from common loquat and Oakleaf loquat.