Development and optimization of a defined medium for aerobic growth ofBacillus stearothermophilus LLD-15 |
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| Authors: | S A Amartey D J Leak B S Hartley |
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| Institution: | (1) Centre for Biotechnology, Imperial College of Science, Technology and Medicine, SW7 2AZ London |
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| Abstract: | Methionine, biotin, nicotinic acid and thiamine were found to be absolute requirements for growth ofBacillus stearothermophilus strain LLD-15, an L-lactate dehydrogenase mutant, at 70°C. A defined medium for aerobic growth did not support anaerobic growth. Physiological and subsequent taxonomic studies showed that strain LLD-15 is not a derivative ofB. stearothermophilus NCA 1503 as originally thought, but bears some similarity toB. caldotenax. |
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