| 羊血铜锌超氧化物歧化酶(Cu-ZnSOD)的分离纯化 |
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| 引用本文： | 高巍,孙庆林. 羊血铜锌超氧化物歧化酶(Cu-ZnSOD)的分离纯化[J]. 生物技术, 2010, 20(1): 86-89 |
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| 作者姓名： | 高巍 孙庆林 |
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| 作者单位： | 内蒙古农业大学生物工程学院,内蒙古,呼和浩特,010018 |
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| 摘 要： | 目的:对羊血进行提取SOD。方法:采用有机溶剂去除血红蛋白、热变性、丙酮沉淀、DEAE-32离子交换柱层析的方法,对羊红细胞Cu,Zn-SOD进行分离纯化。利用非变性凝胶电泳NBT活性染色鉴定SOD,SDS-PAGE测定分子量。结果:表明1 000ml羊血中得到SOD干粉1 257mg,总活力为79 453U,比活力为3 871.4U/mg。活性染色结果证明羊血SOD有2条带,表明已达到电泳纯。SDS-PAGE测得SOD亚基分子量分别为16.71kDa、15.97kDa。H2O2对羊血CuZn-SOD活性有抑制作用,通过紫外光谱扫描,羊血SOD在231nm处有最大吸收峰。
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| 关 键 词： | 羊血 超氧化物歧化酶 纯化 凝胶电泳 |
Separation,Purification of Cu,Zn-SOD from Goat Blood |
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| GAO Wei,SUN Qing-lin. Separation,Purification of Cu,Zn-SOD from Goat Blood[J]. Biotechnology, 2010, 20(1): 86-89 |
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| Authors: | GAO Wei SUN Qing-lin |
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| Affiliation: | GAO Wei,SUN Qing-lin(College of Bioengineering,Inner Mongolia Agricultural University,Hubhot 010018,China) |
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| Abstract: | Objective:To extraet SOD from goat blood. Method: Cu, Zn - SOD was purified from goat blood by organic solvent to remove hemoglobinn, thermal denaturalization, super - filtration condensation, acetone precipitation and DEAE - 32 ion - exchange chromatography. using non - denaturing gel electrophoresis, stained with NBT activity and its molecular weight was analyzed by the SDS - PAGE. Retrophoresis showed that SOD has two SOD activity bands. Through SDS - PAGE electrophoresis determination of the two SOD subunits proteins with molecular weight were 16.71kDa,15.97kDa. The purified goat blood SOD activity inhibition by H_2O_2,Through UV scanning,there was goat blood SOD in the 230.5nm absorption peak. |
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| Keywords: | goat blood superoxide dismutase purifying electrophoresis |
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