Dehydroepiandrosterone improves hepatic antioxidant reserve and stimulates Akt signaling in young and old rats |
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Authors: | Jacob Maria Helena Vianna Metello Janner Daiane da Rocha Araújo Alex Sander da Rosa Jahn Matheus Parmegiani Kucharski Luiz Carlos Rios Moraes Tarsila Barros Dutra Filho Carlos Severo Ribeiro Maria Flavia Marques Belló-Klein Adriane |
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Institution: | Laboratório de Fisiologia Cardiovascular, ICBS - UFRGS, Av Sarmento Leite 500, CEP 90050-170 Porto Alegre, Rio Grande do Sul, Brazil. mhvmjacob@hotmail.com |
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Abstract: | This study examined, in the liver of young and old (3- and 24-month-old, respectively) healthy Wistar rats, the in vivo effect of dehydroepiandrosterone (DHEA) (10mg/kg body weight) administered subcutaneously for 5 weeks. Reduced (GSH) and oxidized (GSSG) glutathione levels, glucose-6-phosphate dehydrogenase (G6PDH), glutathione-S-transferase (GST), glutathione peroxidase (GPx) and catalase (CAT) activities, hydrogen peroxide concentration, GST and p-Akt/Akt immunocontent ratio were assessed in hepatic tissue. DHEA treatment significantly increased total glutathione content (17%) and GSH (22%) in 3- and 24-month-old treated groups when compared to control groups. The aging factor increased G6PDH (51%) and GPx (22%) activities as well as the hydrogen peroxide concentration (33%), independently of treatment. DHEA treatment increased p-Akt (54%) and p-Akt/Akt ratio (36%) immunocontents in both treated groups. Increased serum levels of alanine aminotransferase (ALT) in aged rats were reduced by DHEA treatment (34%). |
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