The use of embryo culture for the recovery of plants from cassava (Manihot esculenta Crantz) seeds |
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| Authors: | Brenda J. Biggs Michael K. Smith Kenneth J. Scott |
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| Affiliation: | (1) Department of Biochemistry, University of Queensland, 4067 St Lucia, Qld, Australia |
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| Abstract: | Cassava fertility and seed viability are frequently low, which can be a disadvantage in a breeding programme. An embryo culture method is described whereby embryonic axes are excised from mature seeds and placed on a culture medium containing 1.23  M indolebutyric acid (IBA) at 30°C under continuous light. The number of plants recovered by embryo culture was much greater than the number recovered from conventional seed germination procedures. |
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| Keywords: | Manihot esculenta cassava embryo culture seed germination |
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