| 基因枪介导甘蔗遗传转化几个影响因素的研究 |
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| 引用本文： | 秦新民,叶云,于兰.基因枪介导甘蔗遗传转化几个影响因素的研究[J].广西植物,2003,23(4):339-342. |
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| 作者姓名： | 秦新民 叶云 于兰 |
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| 作者单位： | 广西师范大学生命科学学院,广西,桂林,541004 |
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| 基金项目： | 广西自然科学基金资助项目(20007007) |
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| 摘 要： | 用基因枪GJ1000介导将Bt基因转入甘蔗嫩叶、Ⅰ型愈伤组织和Ⅱ型愈伤组织。结果表明,用Ⅱ型愈伤组织作为受体最用利于转化;气体压力、轰击距离、轰击次数和真空度对转化效率有不同程度的影响。条件优化后,得到了大量的抗性愈伤组织和一些抗性植株,转化率分别为34.9％和3.36％。
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| 关 键 词： | 甘蔗 基因枪 遗传转化 抗性植株 |
| 文章编号： | 1000-3142(2003)04-0339-04 |
| 修稿时间： | 2002年12月2日 |
Factors affecting microprojectile bombardment- mediated transformation of sugarcane (Saccharum officinarum) |
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| QIN Xin-min,YE Yun,YU Lan.Factors affecting microprojectile bombardment- mediated transformation of sugarcane (Saccharum officinarum)[J].Guihaia,2003,23(4):339-342. |
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| Authors: | QIN Xin-min YE Yun YU Lan |
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| Abstract: | Tender leaves, Ⅰ -type calli and Ⅱ -type calli were transformed with Bt gene by Microprojectile bombardment GJ1000. The results showed that II -type calli as receptor was propitious to transformation. The efficiency of transformation was varied with gas pressure, distance of bombardment, time of bombardment and degree of vacuum. After optimizing these factors,a large number of resistant calli and some resistant plantlets were obtained. The efficiency of transformation are 34. 9% and 3. 36% respectively. |
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| Keywords: | sugarcane microprojectile bombardment genetic transformation resistant plantlet |
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