Characterization of Neurospora crassa mutant stratins deficient in adenylate cyclase activity. |
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| Authors: | M M Flawiá H F Terenzi H N Torres |
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| Affiliation: | 1. Instituto de Investigaciones Bioquímicas “Fundación Campomar” and Facultad de Ciencias Exactas y Naturales, Buenos Aires (28), Argentina;2. Department of Molecular Biology, The Wellcome Research Laboratories, Burroughs Wellcome Company, 3030 Cornwallis Road, Research Triangle Park, N.C. 27709 USA |
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| Abstract: | Mycelial extracts from Neurospora crassa strains having any one of five different mutations of the cr-1 allele (“crisp”) exhibited an adenylate cyclase specific activity 2 to 3% of that found in wild-type strains. The enzyme deficiency seemed to be specific for the cr-1 mutation but not for the “crisp” morphology and recessive in heterocaryons carrying mutated and wild-type cr-1 alleles. The reduced adenylate cyclase activity detected in extracts from cr-1 mutants was not due to an impairment in the extraction of membranes, to a preferential inactivation of the enzyme after extraction, or to the presence of inhibitors. |
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| Keywords: | To whom all correspondence should be addressed at Burroughs Wellcome. |
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