Wheat germ agglutinin binding sites on human urothelial cells of different grades of transformation |
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| Authors: | Danuta Dus Maciej Ugorski Wojciech Gorczyca Czeslaw Radzikowski |
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| Institution: | (1) Department of Tumor Immunology, Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, 53114 Wroclaw, Poland;(2) Department of Immunochemistry, Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, 53114 Wroclaw, Poland |
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| Abstract: | 125I-Wheat germ agglutinin (WGA) binding parameters of human urothelial cell lines of different grades of transformation (TGrll and TGrlll) were compared. The values of association constant (Ka) and the number of binding sites/cell for HCV29 (TGrll) cell line were about 3×106M–1 and over 4×107, respectively. Two TGrlll cell lines, HCV29T and Hu549 revealed lower values for Ka, and considerably higher numbers of binding sites/cell (about 3×108 and 2×108, respectively). Binding of125I-WGA to total cellular proteins resolved by SDS-PAGE and transferred to nitrocellulose showed multiple diffused bands in the range of 58–180 kDa. Some of these bands were characteristic for TGrll cells (124 kDa) or TGrlll cells (135 and 148 kDa).Abbreviations TGr
transformation grade
- WGA
wheat germ agglutinin
- sWGA
succinylated wheat germ agglutinin
- GlcNAc
N-acetyl-d-glucosamine
- BSA
bovine serum albumin
- SDS-PAGE
sodium dodecyl sulphate polyacrylamide gel electrophoresis |
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| Keywords: | transformation grade wheat germ agglutinin Scatchard plots membrane glycoproteins |
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