Adult rat hepatocytes cultured on an oxygen-permeable film increases the activity of albumin secretion |
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Authors: | Hiroshi Kurosawa Ruri Yasuda Yu-ki Osano Yoshifumi Amano |
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Institution: | (1) Applied Chemistry and Biotechnology, Faculty of Engineering, Yamanashi University, Takeda, Kofu, Yamanashi 400-8511, Japan;(2) Applied Chemistry and Biotechnology, Faculty of Engineering, Yamanashi University, Takeda, Kofu, Yamanashi 400-8511, Japan |
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Abstract: | Primary culture of rat hepatocyte was performed in an oxygen-permeable film dish (F-dish), which would be expected to give
an oxygen-rich culture condition. In the conventional culture dish in which the depth of medium was 2 mm, the oxygen tension
(pO2) in the medium decreased from 19% (144 mmHg) to 0.3% (2.3 mmHg) within 2 hr, while the pO2 in the F-dish maintained 8.5% (64.6 mmHg) even after 2 hr. The adverse effect of the oxygen-deficiency appeared in the albumin
secretion activity of the hepatocytes and it was more remarkable in the early period of culture. The average rate of albumin
secretion for the initial 48 hr was 2.0 μg ml-1 hr-1 or 96 μg 106 cells-1 day-1 in the F-dish. The average rate of albumin secretion for the initial 12 hr was only 0.36 μg ml-1 hr-1 in the conventional culture dish. The activity of ammonia elimination in the F-dish was 20–50% higher than the conventional
culture dish. Three-dimensional aggregate was formed only in the F-dish. The advantage of three-dimensional aggregate for
albumin secretion was not clear compared with two-dimensional monolayer.
This revised version was published online in July 2006 with corrections to the Cover Date. |
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Keywords: | albumin cell aggregation hepatocyte oxygen PTFE film |
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