Identification and distribution of actin in spermatogenic cells and spermatozoa of the rabbit |
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| Authors: | J E Welch M G O'Rand |
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| Affiliation: | 1. Department of Chemistry, Charlottesville, VA 22904, USA;2. Department of Mechanical and Aerospace Engineering, Charlottesville, VA 22904, USA;3. Department of Pathology, University of Virginia, Charlottesville, VA 22904, USA;1. Division of Applied Functional Genomics, German Cancer Research Center (DKFZ) and National Center for Tumor Diseases (NCT) Heidelberg, 69120 Heidelberg, Germany;2. Division of Molecular Embryology, DKFZ-ZMBH Alliance, 69120 Heidelberg, Germany;3. Core Facility Electron Microscopy, DKFZ, 69120 Heidelberg, Germany;4. Molecular Biology of Centrosomes and Cilia Unit, DKFZ-ZMBH Alliance, 69120 Heidelberg, Germany;5. Institute of Molecular Biology (IMB), 55128 Mainz, Germany;6. Department of Internal Medicine III, Ulm University, 89081 Ulm, Germany;7. Department of Developmental Biology, Centre for Organismal Studies (COS), 69120 Heidelberg, Germany;8. Division of Translational Oncology, National Center for Tumor Diseases (NCT) Heidelberg and German Cancer Research Center (DKFZ), 69120 Heidelberg, Germany;9. Section for Personalized Oncology, Heidelberg University Hospital, 69120 Heidelberg, Germany;10. German Cancer Consortium (DKTK), 69120 Heidelberg, Germany;1. Department of Mechanical and Aerospace Engineering, University of Missouri, Columbia, MO 65211, USA;2. CryoCrate LLC, 1601 S. Providence Rd., Columbia, MO 65211, USA;1. Veterinary Reproduction Group, Department of Animal Medicine and Surgery, University of Cordoba, Cordoba, 14071, Spain;2. Department of Theriogenology, Faculty of Veterinary Medicine, Assiut University, Assiut, Egypt;3. Departamento de Química Analítica, Instituto Universitario de Investigación en Química Fina y Nanoquímica IUNAN, Universidad de Córdoba, Campus de Rabanales, Edificio Marie Curie, E-14071, Córdoba, Spain;4. College of Veterinary Medicine & Biomedical Sciences, Texas A&M University, College Station, TX, 77843-4466, United States |
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| Abstract: | ![]()
Using a monoclonal antibody as a highly specific probe and a seminal particle-free fraction of rabbit ejaculated spermatozoa, actin has been localized in the postacrosomal region of mature rabbit spermatozoa. The sperm actin has been extracted and identified on two-dimensional PAGE immunoblots as a single spot of pI = 5.45 and Mr = 43,000. Rabbit sperm actin is present in a nonfilamentous form and is not removed by removing the plasma membrane. Unlike mature spermatozoa, however, filamentous actin is present in spermatogenic cells, as determined by rhodamine phalloidin staining. Starting as diffusely distributed in spermatocytes, actin accumulates in the subacrosomal space and appears as a band in conjunction with the developing acrosome. This band lengthens throughout the spermatid stage and becomes continuous with the postacrosomal region staining in testicular spermatozoa. Actin may therefore function during spermatogenesis to both shape the acrosome to the nucleus and to anchor inner acrosomal membrane proteins. |
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