| 表皮形态发生素对肝细胞癌SK-HEP-1细胞生物学活性的影响 |
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| 引用本文： | 傅春江,贾雅丽,周军年,陈琳,曾泉,南雪,岳文,尉承泽,裴雪涛.表皮形态发生素对肝细胞癌SK-HEP-1细胞生物学活性的影响[J].生物技术通讯,2013(6):792-796. |
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| 作者姓名： | 傅春江 贾雅丽 周军年 陈琳 曾泉 南雪 岳文 尉承泽 裴雪涛 |
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| 作者单位： | [1]北京房山区中医院,北京102400 [2]军事医学科学院野战输血研究所,全军干细胞与再生医学重点实验室,北京100850 [3]军事医学科学院附属医院外科,北京100071 |
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| 基金项目： | 国家自然科学家基金（81201611,81201874.81101812） |
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| 摘 要： | 目的：明确表皮形态发生素（EPM）对盱细胞癌SK-HEP-1细胞生物学行为的影响。方法：构建高表达EPM的SK-HEP-1细胞，real-timePCR和Westem印迹检测EPM在肿瘤细胞内的表达，CCK8分析和克隆形成实验检测细胞的增殖能力，Matrigel-transwell实验检测细胞的浸润能力。结果：EPM在肿瘤细胞内的高表达不影响细胞的增殖能力，怛明显增强肿瘤细胞的浸润能力。结论：肝癌肿瘤微环境有可能通过EPM影响肿瘤细胞的生物学活性，对其作用机制的进一步明确，将有助于阐明肝癌发生发展的病理机制，发现新的恶性肿瘤诊断和治疗手段。
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| 关 键 词： | 表皮形态发生素 肝细胞癌 增殖 浸润 |
Effects of Epimorphin on SK-HEP-1 Cell Biology |
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| FU Chun-Jiang,JIA Ya-Li,ZHOU Jun-Nian,CHEN Lin,ZENG Quan,NAN Xue,YUE Wen,YU Cheng-Ze,PEI Xue-Tao.Effects of Epimorphin on SK-HEP-1 Cell Biology[J].Letters in Biotechnology,2013(6):792-796. |
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| Authors: | FU Chun-Jiang JIA Ya-Li ZHOU Jun-Nian CHEN Lin ZENG Quan NAN Xue YUE Wen YU Cheng-Ze PEI Xue-Tao |
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| Institution: | . ( 1. Fangshan Traditional Chinese Medicine Hospital, Beijing 102400; 2. Stem Cells and Regenerative Medicine Lab, Beijing Institute of Transfusion Medicine, Beijing 100850; 3. Department of Hepatobihary Surgery, Affiliated Hospital, Academy of Military Medical Sciences, Beijing 100071; China.) |
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| Abstract: | Objective： To study whether epimorphin（EPM） affect SK-HEP-1 cell biology. Methods： SK-HEP-1 ceils with ectopic EPM expression were constructed and identified by real-time PCR and Western blot. CCK8 analysis and colony formation assay were used to detect cancer cell proliferation. The invasion activity of cancer cells were detected by Matrigel-transwell assay. Results： We first confirmed with that EPM was unregulated in hepatocellular carcinoma（HCC） tumor stroma tissues. And, the further results suggested that ectopic expression of EPM in SK-HEP-1 cell had no effects on cancer cell proliferation, but enhanced the cell invasion activity significantly. We also showed that EPM could enhance FAK activation significantly. Conclusion： Taken together, our results re- veal that EPM was involved in HCC progression and may be a potential therapeutic target for HCC. |
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| Keywords: | epimorphin hepatocellular carcinoma proliferation invasion |
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