响应面法优化提取虎耳草总酚及其抑制五步蛇毒PLA2酶活性的相关性

为了探讨虎耳草总酚（TP）与抑制五步蛇毒中磷脂酶A₂（PLA₂）之间的关系，本研究通过单因素试验及Box-Behnken试验对虎耳草TP的提取条件与抑制五步蛇毒中PLA₂活性进行优化，考察各试验因素对超声辅助提取虎耳草TP得率及其对五步蛇毒PLA₂活性抑制率（PIR）的作用，并对虎耳草TP含量与PIR的相关性进行分析。结果表明，响应面法提取虎耳草TP和PIR的最佳工艺条件为：粒径为60目，料液比为1∶50，超声功率为250 W，超声时间为1.8 h。此优化工艺条件下虎耳草TP得率为（8.69±0.46） mg·g^-1，PIR为（40.91±0.21）%；相关性分析结果显示虎耳草TP含量与PIR具有极显著正相关（P<0.01），说明TP可能为虎耳草抑制五步蛇毒中PLA₂活性的物质基础。

Optimization on Extraction of Total Phenols by Response Surface from Saxifraga stolonifera and Its Correlation in Inhibiting the Activity of PLA2 in Deinagkistrodon acutus Venom

To reveal the relationship between total phenols(TP)of Saxifraga stolonifera and inhibition of the activity of phospholipase A₂(PIR)in Deinagkistrodon acutus venom,we used the single factor and Box-Behnken methods to optimize the extraction conditions of TP of Saxifraga stolonifera and PIR.The effect of various experimental factors on the yield of TP and PIR in the extraction of S.stolonifera by ultrasonic assisted extraction were investigated respectively,and the correlation between the content of TP and PIR were analyzed.The results showed that when the particle size was 60 mesh,the material to liquid ratio was 1∶50,the ultrasonic power was 250 W,and the ultrasonic time was 1.8 h,the content of TP in the aqueous extract of S.stolonifera and its inhibitory rate(PIR)of the PLA₂ activity in D.acutus venom could reach the optimal ultrasonic-assisted extraction process conditions.The content of TP was(86.90±0.46)mg·g^-1,and the PIR was(40.91±0.21)%.The results of correlation analysis showed that the content of TP and the PIR were extremely significant correlation(P<0.01),indicating that the TP might be the material basis of S.stolonifera to inhibit the PLA₂ activity in D.acutus venom.