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Production of a Cellulase in Silkworm Larvae using a Recombinant Bombyx mori nucleopolyhedrovirus lacking the Virus-encoded Chitinase and Cathepsin Genes |
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| Authors: | Kwang Sik Lee Yeon Ho Je Soo Dong Woo Hung Dae Sohn Byung Rae Jin |
| Institution: | (1) College of Natural Resources and Life Science, Dong-A University, 604-714 Busan, Korea;(2) School of Agricultural Biotechnology, Seoul National University, 151-742 Seoul, Korea;(3) Department of Plant Medicine, Chungbuk National University, 361-763 Cheongju, Korea |
| Abstract: | The expression efficiency of an insect-derived cellulase was assayed in silkworm larvae infected with recombinant Bombyx mori nucleopolyhedrovirus (BmNPV) mutants lacking the virus-encoded chitinase (chiA) and/or cathepsin (v-cath) genes. Expression was increased by approx. 10% in mutants lacking chiA or v-cath and 17% in a mutant lacking both chiA and v-cath compared with that of the unmodified recombinant BmNPV. The recombinant BmNPV lacking both chiA and v-cath can therefore be used for a large-scale production of foreign proteins in silkworms. Revisions requested 27 September 2005 and 16 December 2005; Revisions received 6 December 2005 and 3 February 2006 |
| Keywords: | baculovirus expression vector Bombyx mori nucleopolyhedrovirus cathepsin cellulase chitinase silkworm |
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