An improved micropropagation of <Emphasis Type="Italic">Eclipta alba</Emphasis> by in vitro priming with chlorocholine chloride |
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Authors: | Avik Ray Sabita Bhattacharya |
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Institution: | (1) Department of Botany, Bose Institute, 93/1, A.P.C. Road, Kolkata, 700 009, West Bengal, India |
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Abstract: | An efficient method of micropropagation for Eclipta alba from young nodal axils of shoot tip explants has been developed by giving special attention to ‘priming’ in vitro plantlets
in view of increasing their hardening ability after transplantation ex vitro. Among 3 cytokinins—BAP, kinetin and TDZ, BAP
was found most effective in inducing and proliferating adventitious shoots. The highest frequency of responding explants (100%)
and maximum number of shoots (23.0) per explant were obtained after 60 days culture on MS medium containing 8.8 μM BAP. Cent
percent shoots developed roots directly from shoot base when transferred to growth regulator-free MS medium. For priming E. alba microshoots, 6.3 μM of chlorocholine chloride (CCC) was found most effective. The major changes observed in 30 days old treated
shoots were, production of increased number of root, elevation of chlorophyll level in leaves and increase in plant biomass.
Furthermore, arrested undesirable shoot elongation made the plants sturdier and more suitable for acclimatization. The primed
micropropagated E. alba plants were healthy and survived by higher frequency (100%) in soil in comparison to the non-treated plants (84% survival). |
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Keywords: | Eclipta alba Micropropagation Shoot multiplication Cytokinin Priming Growth retardant |
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