避暗反应测定大鼠学习记忆功能方法的探讨

目的 为了提高避暗反应测定动物学习记忆功能的敏感性,本实验对三种实验及统计方法进行了评价。方法 采用IBO致基底前脑胆碱能神经元损伤模型和东莨菪碱所致的记忆障碍模型,对单次简单测试法、多次简单测试法和多次记分测试法的敏感性进行了比较。结果 对于基底前脑损伤模型,单次简单测试法的潜伏期学习记忆指标无法显示出模型组与对照组之间的差异。多次简单测试法两种指标均能显示出显著性差异,且较多次记分测试法的指标差异的显著性高。对于东莨菪碱致痴呆模型,也有同样的效果。结论 多次简单测试法更能敏感地反映动物的学习记忆功能。

Exploration of Step Through Avoidance Response: Determination of Learning and Memory Function in Rat

Objective To compare three tests and statistical methods in the measurement sensitivity of learning and memory function in rat by step through avoidance response. Methods Learning and memory dysfunction rat models caused by injuring neuron of basal forebrain and injecting scopolamine were adopted and studied on by three methods of simple mono-test,simple multi-test and scoring multi-test. Results For learning and memory dysfunction rat model caused by injuring neuron of basal forebrain, latency time could not indicate significant difference between model and control by simple mono-test, whereas the two kinds of indication in simple multi-test could.The latter was also better than scoring multi-test. The results for learning and memory dysfunction rat model caused by injecting scopolamine were similar to models above. Conclusion Simple multi-test is the most sensitive method for measuring learning and memory in animal by step through voidance response.