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Expression and characteristic of synthetic human epidermal growth factor (hEGF) in transgenic tobacco plants |
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| Authors: | Jie-Ying Bai Lin Zeng Yuan-Lei Hu Yan-Fang Li Zhong-Ping Lin Shi-Chen Shang Yan-Sheng Shi |
| Affiliation: | (1) The National Laboratory of Protein Engineering and Plant Genetic Engineering, Peking University, Beijing, 100871, P.R. China;(2) Laboratory Animal Center of the Academy of Military Medical Science, Beijing, 100071, P.R. China;(3) College of Plant Sciences, Jilin University, Changchun, 130062, P.R. China |
| Abstract: | To improve the accumulation of recombinant human epidermal growth factor (hEGF) in transgenic tobacco, a highly effective vector was constructed and transformed via Agrobacterium tumefaciens. The hEGF content in transgenic tobacco was up to 0.3% of the total soluble protein. Using the Vero E6 cell expansion assay and the MTT method for cell proliferation, hEGF produced by transgenic tobacco significantly stimulated Vero E6 cell expansion and proliferation, the same as commercial hEGF products. |
| Keywords: | Biological activity Human epidermal growth factor Matrix attachment regions Optimized codons Tobacco |
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