| 右旋糖酐酶产生菌的筛选及分类鉴定 |
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| 引用本文： | 岳晓婧,戴玲,沈业寿. 右旋糖酐酶产生菌的筛选及分类鉴定[J]. 生物学杂志, 2011, 28(1): 10-13,5. DOI: 10.3969/j.issn.1008-9632.2011.01.010 |
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| 作者姓名： | 岳晓婧 戴玲 沈业寿 |
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| 作者单位： | 安徽大学生命科学学院,合肥,230039 |
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| 基金项目： | ,安徽大学211工程学术创新团队项目资助, |
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| 摘 要： | 从土壤中筛选出一株右旋糖酐酶产生菌（D5）,可水解右旋糖酐产生单一产物——异麦芽三糖,为外切型异麦芽三糖水解酶产生菌。经分生孢子、分生孢子梗、菌落形态,色素颜色等形态学观察,分析其为黄绿青霉。对菌株的ITS rDNA序列进行克隆测序,与GenBank中已知菌的ITS rDNA比对,用Neighbor-joining方法构建聚类分析树状图,并用Bootstrap法对其评估,结果表明ITS序列的分子鉴定支持了基于形态特征的鉴定结果。该菌株的ITS与Penicillium daleae,Penicillium janthinellum菌株的ITS rDNA序列同源性最高。
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| 关 键 词： | 右旋糖酐酶 异麦芽三糖 青霉 ITS 分类鉴定 |
Screening and identification of a dextranase yield fungus |
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| YUE Xiao-jing,DAI Ling,SHEN Ye-shou. Screening and identification of a dextranase yield fungus[J]. Journal of Biology, 2011, 28(1): 10-13,5. DOI: 10.3969/j.issn.1008-9632.2011.01.010 |
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| Authors: | YUE Xiao-jing DAI Ling SHEN Ye-shou |
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| Affiliation: | YUE Xiao-jing,DAI Ling,SHEN Ye-shou (School of Life Science,Anhui University,Hefei 230039,China) |
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| Abstract: | An exo-isomaltotriodextranase yield strain was screened.It showed higher activity against alpha-(1,6) glucan and released isomaltotriose as main products from dextran 70000.After observation of morphology and color of the colony,morphology of conidiospores and conidiophores,the strain was preliminarily identified as Penicillium citreoviride.The ITS rDNA was cloned and sequenced,and then was compared with those available in the GenBank databases.The phylogenetic trees were derived with neighbor joining and a... |
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| Keywords: | ITS |
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