电场对完整青蛙横纹肌收缩机理的椭圆偏振法研究

利用能反映肌纤维内部结构的椭圆偏振参数研究恒定电场，脉冲电场对完整的青蛙横纹肌收缩的影响，研究表明，电场对肌纤维作用后，肌质网释放钙离子，致使肌纤维中蛋白质分子运动后出现收缩效应。实验发现：椭圆偏参数的测量灵敏度和肌小节长度有关，蛋白质运动规律和电场的性质的关系无的差异，只有量的不同。它们的共同特点是，随着电场强度的逐渐增强，其退偏率的变化呈先下降一上升的凹形曲线，这是由于电场引起的肌球蛋白分子极

STUDY ON THE CONTRACTION MECHANISM OF THE FROG INTACT MUSCLE FIBRES STIMULATED BY THE ELECTRIC FIELD USING THE ELLIPSOMETRIC METHOD

The effect of the static or pulsed electric field on the contraction of the frog intact muscle fibres is investigated. It is very clear when the electric field stimulates the intact muscle fibres, the SR releases the Ca ions, which could make the muscle contract. The experiments show that the sensitivity of the ellipsometric parameters depend on the sarcomere length, the relationships between the motion of the cross -bridge and different types of the electric field (static or pulsed) are same in quality, but different in quantity, and depolarization ratio is a function of the electric field strength. both in static or pulsed. The later result is raised by the competition of two mechanisms; one is electric attractive force between the LMM and HMM. which are polarized by the electric feild, and the another one is the force from the weakbinding cross -bridge transfering to the strong binding cross -bridge due to the Ca ions .