Selective enrichment,isolation and molecular detection of <Emphasis Type="Italic">Salinibacter</Emphasis> and related extremely halophilic <Emphasis Type="Italic">Bacteria</Emphasis> from hypersaline environments |
| |
Authors: | Rahel Elevi Bardavid Danny Ionescu Aharon Oren Fred A Rainey Becky J Hollen Danielle R Bagaley Alanna M Small Christopher McKay |
| |
Institution: | (1) The Institute of Life Sciences, and The Moshe Shilo Minerva Center for Marine Biogeochemistry, The Hebrew University of Jerusalem, Jerusalem, 91904, Israel;(2) Department of Biological Sciences, Louisiana State University, Baton Rouge, LA 70803, USA;(3) Space Science Division, NASA-Ames Research Center, Moffett Field, CA 94035, USA |
| |
Abstract: | Salinibacter is a genus of red, extremely halophilic Bacteria. Thus far the genus is represented by a single species, Salinibacter ruber, strains of which have been isolated from saltern crystallizer ponds in Spain and on the Balearic Islands. Both with respect
to its growth conditions and its physiology, Salinibacter resembles the halophilic Archaea of the order Halobacteriales. We have designed selective enrichment and isolation techniques to obtain Salinibacter and related red extremely halophilic Bacteria from different hypersaline environments, based on their resistance to anisomycin and bacitracin, two antibiotics that are
potent inhibitors of the halophilic Archaea. Using direct plating on media containing bacitracin, we found Salinibacter-like organisms in numbers between 1.4×103 and 1.4×106ml−1 in brines collected from the crystallizer ponds of the salterns in Eilat, Israel, being equivalent to 1.8–18% of the total
colony counts obtained on identical media without bacitracin. A number of strains from Eilat were subjected to a preliminary
characterization, and they proved similar to the type strain of S. ruber. We also report here the isolation and molecular detection of Salinibacter-like organisms from an evaporite crust on the bottom of salt pools at the Badwater site in Death Valley, CA. These isolates
and environmental 16S rRNA gene sequences differ in a number of properties from S. ruber, and they may represent a new species of Salinibacter or a new related genus.
Guest Editor: John M. Melack
Saline Waters and their Biota |
| |
Keywords: | Salinibacter Salterns Death Valley Hypersaline Enrichment Anisomycin Bacitracin |
本文献已被 SpringerLink 等数据库收录! |
|