| 肥皂草素类核糖核蛋白体失活蛋白的纯化及其性质研究 |
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| 引用本文： | 沈倍奋,白炎.肥皂草素类核糖核蛋白体失活蛋白的纯化及其性质研究[J].中国生物化学与分子生物学报,1990,6(4):301-305. |
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| 作者姓名： | 沈倍奋 白炎 |
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| 作者单位： | 军事医学科学院基础医学研究所 北京
(沈倍奋)，军事医学科学院基础医学研究所 北京(白炎) |
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| 摘 要： | 利用强阳离子交换柱从Saponaria officinalis L种子中分离出一种肥皂草素(Saporin)成分。它在无细胞体系中显示了较强的抑制蛋白合成的活性,与抗体连接后能特异性杀伤靶细胞。
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| 关 键 词： | 肥皂草素 免疫毒素 核糖核蛋白体失活蛋白 |
| 收稿时间： | 1990-08-20 |
Purification and Characterization of a ribosome inactivating proteins,Saporin |
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| Shen,Bei-fen Bai,Yan.Purification and Characterization of a ribosome inactivating proteins,Saporin[J].Chinese Journal of Biochemistry and Molecular Biology,1990,6(4):301-305. |
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| Authors: | Shen Bei-fen Bai Yan |
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| Institution: | (Institute of Basic Medical Sciences, Academy of Military Medical Sciences, Beijing |
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| Abstract: | A new ribosome-inactivating protein SL was purified from the seeds of Saponaria officinalis by strong cation exchange chromatography. In a rabbit reticulo-cyte lysate test system, the purified protein SL caused 50% inhibition of protein synthesis at a concentration of 1×10-12mol/L. After coupling to monoclonal anti-thy 1.1 antibody (OX7) , the immunotoxin showed enhanced cytotoxic effect to target cells. |
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| Keywords: | Saporin immunotoxin ribosome-inactivating protein |
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