Combined influence of light and temperature on growth rates of <Emphasis Type="Italic">Nannochloropsis oceanica</Emphasis>: linking cellular responses to large-scale biomass production |
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Authors: | Email author" target="_blank">J?M?SandnesEmail author T?K?llqvist D?Wenner H?R?Gisler?d |
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Institution: | 1.Norwegian University of Life Sciences (UMB), Dept. of Plant and Environmental Sciences,Oslo,Norway;2.Norwegian Instiute for Water Research (NIVA),Oslo,Norway;3.Norwegian University of Life Sciences (UMB), Centre for Plant Research in Controlled Climate,Oslo,Norway |
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Abstract: | The interaction effects between irradiance and temperature on growth rates ofNannochloropsis oceanicawere determined in both laboratory cultures and large-scale tubular photobioreactors. Growth responses were investigated in
48 batch cultures subjected to crossing light/temperature gradients ranging from 34–80μmol photons m−2s−1and 14.5–35.7∘C respectively. Comparisons were made to growth responses observed in production systems (200L biofences) operated in climate-regulated
greenhouses with controlled temperature and artificial light gradients. Cellular responses showed increasing specific growth
rates as a function of temperature, with a peak at 25–29∘C, after which the growth became increasingly unstable. The optimum temperature for growth increased with higher light intensities
up to approximately 28∘C at 80μmol photons m−2s−1. At low light intensities the specific growth rate was less affected by temperature. The maximum daily production measured
in the biofence systems increased proportionally with irradiation and reached approximately 0.7gL−1d−1at 1030μmol photons m−2s−1average daily radiation for a culture temperature of 24∘C. This corresponds to a daily yield of 140g per day in a 200L biofence system. When specific growth rates for the biofence
cultures were measured at different densities and plotted against temperature, results showed a peak with the 24∘C temperature treatment. This peak became less pronounced as the density increased in the cultures. This is consistent with
the laboratory results; increasing cell density in the biofence cultures resulted in less average light cell−1, which produced the same temperature dependent response as seen by reducing the external irradiance exposure for the dilute
laboratory cultures. |
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Keywords: | algae artificial lighting biomass greenhouses microalgae photobioreactor |
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