Genetic diversity of durum wheat as determined by AFLP in fluorescence |
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| Authors: | F Sciacca C Fichera S Di Silvestro E Conte and M Palumbo |
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| Institution: | (1) Istituto Nazionale di Ricerca per gli Alimenti e la Nutrizione, via Ardeatina, 546 00178 Rome, Italy;(2) Sezione di Industrie agro-alimentari, Dipartimento PRO.GE.S.A, Università degli Studi di Bari, Via Amendola 165/A, 70126 Bari, Italy;(3) Dipartimento di Biologia e chimica agro-forestale ed ambientale, Sezione di Genetica e miglioramento genetico, Università degli Studi di Bari, Via Amendola 165/A, 70126 Bari, Italy |
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| Abstract: | The DNA of fifteen Italian cultivars of durum wheat (Triticum turgidum L. ssp. durum) were analyzed by in fluorescence amplified fragment length polymorphism (fAFLP) in order to obtain the characteristic fingerprintings
of genotypes and assess their genetic relatedness. Among 64 combinations of fluorescence labelled primers, three different
combinations were chosen as producing a total of 6630 AFLP fragments, 2277 (34.3 %) of them being polymorphic. By using this
fAFLP methodology a DNA fingerprinting of each durum wheat cultivar was generated for genotype identification. Analysis of
the genetic relationships show the low variability among durum wheat cultivars. |
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