Dynamic thiol/disulphide homeostasis as indicator of oxidative stress in automotive workers |
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Authors: | Murat Büyük?ekerci Me?ide Gündüzöz Osman Gökhan Özak?nc? Mevlüt Karata? Almila ?enat Salim Ne?elio?lu |
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Institution: | 1. Department of Pharmacology, Occupational and Environmental Diseases Hospital, Ankara, Turkey;2. drmuratbs@gmail.com;4. Department of Family Medicine, Occupational and Environmental Diseases Hospital, Ankara, Turkey;5. Department of Public Health, Occupational and Environmental Diseases Hospital, Ankara, Turkey;6. Department of Chest Diseases, Occupational and Environmental Diseases Hospital, Ankara, Turkey;7. Department of Biochemistry, Y?ld?r?m Beyaz?t University, Ankara, Turkey |
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Abstract: | AbstractPurpose: To examine thiol-disulphide homeostasis auto painters.Materials and methods: A total of 115 male workers, including 60 auto painters workers and 55 reference group, of the painting and assembly line units respectively, were included in the study. Thiol-disulphide parameters and ischaemia-modified albumin (IMA) of groups were determined. Urinary hippuric acid, (HA) phenol, hexanedione, trichloroacetic acid, arsenic and blood lead and manganese were analysed.Results: The median urinary HA level was significantly higher in auto painters when compared to the reference group (2461 (1212) vs. 520 (513) µgr/L), (p?<?0.001)] . The mean disulphide level 19.7 (4.3) vs 0.15.1(4.1) μmol/L, (p?<?0.001)], the disulphide/native thiol ratio 4.72 (1.47) vs. 3.13 (1.21, (p?<?0.001)] and the disulphide/total thiol ratio 4.31 (1.23) vs. 2.94 (1.06), (p?<?0.001)] were higher in auto painters when compared to the reference group. There was a statistically significant positive correlation between urinary HA and disulphide concentrations (r?=?0.536 and p?<?0.001), disulphide/native thiol ratio (r?=?0.564 and p?<?0.001) and the disulphide/total thiol ratio (r?=?0.564 and p?<?0.001) and IMA (r?=?0.396 and p?<?0.001).Conclusion: The results presented in this study showed that oxidative stress can be associated with occupational exposure to toluene denoted by alteration of thiol disulphide homeostasis and ischaemia-modified albumin levels. |
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Keywords: | Auto painters toluene thiol disulphide ischaemia-modified albumin oxidative stress |
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