Highly regioselective methylation of inosine nucleotide: an efficient synthesis of 7-methylinosine nucleotide |
| |
Authors: | Annamalai Senthilvelan Muthian Shanmugasundaram |
| |
Institution: | Life Sciences Solutions Group, Thermo Fisher Scientific, Austin, Texas, USA |
| |
Abstract: | AbstractA facile, straightforward, reliable, and an efficient chemical synthesis of inosine nucleotides such as 7-methylinosine 5′-O-monophosphate, 7-methylinosine 5′-O-diphosphate, and 7-methylinosine 5′-O-triphosphate, starting from the corresponding inosine nucleotide is delineated. The present methylation reaction of inosine nucleotide utilizes dimethyl sulfate as a methylating agent and water as a solvent at room temperature. It is noteworthy that the present methylation reaction proceeds smoothly under aqueous conditions that is highly regioselective to afford exclusive 7-methylinosine nucleotide in good yields with high purity (>99.5%). |
| |
Keywords: | Inosine 7-methylinosine nucleotide methylation regioselective |
|
|