Cloning and characterization of PhPI9 involved in floral development from Phalaenopsis Orchid |
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| Authors: | Bin Guo Wei Dai Donghong Chen Xing Wei Feng Ming |
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| Institution: | 1. Institute of Genetics, State Key Laboratory of Genetic Engineering, Research Center of Gene Diversity and Designed Agriculture, Ministry of Education Key Laboratory for Biodiversity Science and Ecological Engineering, School of Life Science, Fudan University, Shanghai, 200433, China
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| Abstract: | In the attempt to discover new genes involved in the floral development in monoeotyledonousin species,we have cloned and characterized the homologous PISTALLATA-like (PI-like) gone from Phalaenopsis hybrid cultivar named PhPI9 (Phalaenopsis PI STILLATA # 9).The eDNA of PhPI9 has a fragment of 834 bp and has 60% identity with the PISTILATA from Arabidopsis.The deduced amino acid sequence of PhPI9 had the typical PI-motif.It also formed a subelade with other monoeot PI-type genes in phylogenetie analysis.Southern analysis showed that PhPI9 was present in the Phalaenopsis orchid genome as a single copy.Furthermore,it was expressed only in the lip of the Phalaenopsis flower and no expression was detected in vegetative organs.Thus,as a B-function MADS-box gone,PhP19 specifies floral organ identity in orchids. |
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| Keywords: | Phalaenopsis MADS-box gene floral development RT-PCR |
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