| 高效液相色谱法测定17AA氨基酸注射液中N-乙酰-L-半胱氨酸和N-乙酰-L-酪氨酸 |
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| 引用本文： | 陈永波,饶斌,沈艳芬,程群,赵清华,田远政. 高效液相色谱法测定17AA氨基酸注射液中N-乙酰-L-半胱氨酸和N-乙酰-L-酪氨酸[J]. 氨基酸和生物资源, 2002, 24(4): 67-70. DOI: 10.3969/j.issn.1006-8376.2002.04.022 |
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| 作者姓名： | 陈永波 饶斌 沈艳芬 程群 赵清华 田远政 |
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| 作者单位： | 湖北恩施南方马铃薯研究中心,湖北,恩施,445000;湖北省八峰药化股份有限公司,湖北,鹤峰,445800 |
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| 摘 要： | 建立了一种用高效液相色谱测定 17AA氨基酸注射液中N 乙酰 L 半胱氨酸和N 乙酰 L 酪氨酸含量的方法。样品经稀释后 ,直接上机测定 ,其色谱条件为 :WatersSymmetryC18色谱柱 ,流动相为 8.5mmol/LNaAc - 5 %CH3 OH (pH =4.0 ) ,柱温36℃ ,流速 1.0ml/min ,检测波长 195nm ,N 乙酰 L 半胱氨酸在 5 .4mg/L到 5 40mg/L、N 乙酰 L 酪氨酸在 4.9mg/L到 490mg/L之间 ,标准曲线呈良好的线性关系 (r2 >0 .9999)。该方法具有良好的重现性 ,日内相对标准偏差小于 3% ,日间相对平均误差小于 7% ,样品回收率在 90 %～ 110 %之间。该方法与测定氨基酸注射液的其它高效液相色谱法相结合 ,能够对 17AA氨基酸注射液中所有氨基酸成分进行全面检测。
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| 关 键 词： | 高效液相色谱 N-乙酰-L-半胱氨酸 N-乙酰-L-酪氨酸 17AA氨基酸注射液 |
| 文章编号： | 1006-8376(2002)04-0067-04 |
| 修稿时间： | 2002-04-23 |
Analysis of N-Acetyl-L-Cysteine and N-Acetyl-L-Tyrosine in 17 Amino Acid Injection by High Performance Liquid Chromatography |
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| CHEN Yong-bo ,RAO Bin ,SHEN Yan-fen ,CHEN Qun ,ZHAO Qing-hua ,TIAN Yuan-zheng. Analysis of N-Acetyl-L-Cysteine and N-Acetyl-L-Tyrosine in 17 Amino Acid Injection by High Performance Liquid Chromatography[J]. Amino Acids & Biotic Resources, 2002, 24(4): 67-70. DOI: 10.3969/j.issn.1006-8376.2002.04.022 |
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| Authors: | CHEN Yong-bo RAO Bin SHEN Yan-fen CHEN Qun ZHAO Qing-hua TIAN Yuan-zheng |
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| Affiliation: | CHEN Yong-bo 1,RAO Bin 2,SHEN Yan-fen 1,CHEN Qun 1,ZHAO Qing-hua 1,TIAN Yuan-zheng 2 |
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| Abstract: | The chromatography conditions were: Waters Symmetry C18 column(3.9mm i.d.×150mm, 4μm) as solid phase, 8.5mmol/L sodium acetate-5% methyl alcohol (pH=4.0) as liquid phase, column temperature of 36℃, flow rate of 1.0ml/min and the detect wavelength of 195nm. The calibration curves of N-acetyl-L-cysteine from 5.4mg/L to 540mg/L and N-acetyl-L-tyrosine from 4.9mg/L to 490mg/L were linear(r 2>0.9999). The analytical method was shown to be highly reproducible, and the absolute recoveries were within 90%-110%. |
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| Keywords: | high performance liquid chromatogram N-acetyl-L-cysteine N-acetyl-L-tyrosine 17AA injection |
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