高糖高脂饮食联合注射STZ制备2型糖尿病大鼠缺血性心脏病模型

目的：制备2型糖尿病缺血性心脏病大鼠模型。方法：雄性Wistar大鼠20只，随机分为正常组和糖尿病模型组（n=10）。糖尿病模型组给予高糖高脂饮食饲喂4周后，一次性腹腔注射链脲佐菌素40mg/kg，制备糖尿病大鼠模型。每周常规监测血糖、血清胰岛素、体重的变化。糖尿病模型组与正常组大鼠，使用BL-41O生物机能试验系统，每周测定肢体2导联心电图。最后检测血清中的肌酸激酶和乳酸脱氢酶的含量。结果：高糖高脂饲料饲喂4周后胰岛素升高至4．05ng/ml，糖尿病模型组注射SIZ后空腹血糖迅速升高，达到17．9mmol/L。在第14周，糖尿病模型组出现S-T段抬高，并且与正常组比较，血清肌酸激酶（creatine kinase，CK）和乳酸脱氢酶（lactate dehydrogenase，LDH）均升高。结论：本实验成功建立的2型糖尿病缺血性心脏病大鼠模型，为研究糖尿病缺血性心脏病提供理想的动物模型。

Cardiac ischemia in type 2 diabetes mellitus rats induced by high sucrose and high fat diet and STZ treated

Objective： To build a type 2 diabetes mellitus rat model with cardiac ischemia. Methods： Male Wistar rats were fed high sucrose and high fat diet for four weeks and then injected with streptozoticin（STZ）（40 mg/kg . i. p. ）. The levels of fasting blood glucose and serum insulin were monitored every week. The body weights of rats were also measured every week. The blood levels of creatine kinase and lactate dehydrogenase（LDH） were measured following the electrocardiograph used BL-410 biological experiment system. Results： The serum insulin levels of diabetic rats were 4.05 ng/ml after four weeks high sucrose and high fat diet. The fasting blood glucose levels of diabetic rats were 17.9 mmol/L after injection. Compared with normal group, there was obvious change of S-T segment in the electrocardiograph of diabetic group at the fourteenth week. The levels of creatine kinase and lactate dehydrogenase in diabetic group significantly increased in comparison with those in normal group. Conclusion： The cardiac isehemia of diabetic rats model is suitable for investigating cardiac disease of diabetes mellitus.