Circatidal variation in epithelial cell proliferation in the mussel digestive gland and stomach |
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Authors: | B?Zaldibar I?Cancio Email author" target="_blank">I?MarigómezEmail author |
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Institution: | (1) Departments of Zoology and Animal Cell Biology, School of Science and Technology, University of the Basque Country, P.O. Box 644, 48080 Bilbao, Basque Country, Spain |
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Abstract: | Epithelial cell renewal in mussel (Mytilus galloprovincialis, Lmk) digestive gland and stomach was investigated by bromodeoxyuridine (BrdU) immunohistochemistry. Mussels were exposed to 4 mg BrdU/l seawater continuously. Starting at 6 h after treatment, samples were collected every 2 h for 2 days and BrdU labelling was estimated by direct counting at the light microscope, with values being noted per thousand BrdU-positive cells. BrdU-positive reaction was observed in the nuclei of digestive, basophilic, duct and stomach cells, and in haemocytes. Cell renewal in digestive diverticula was synchronised following a circatidal pattern: BrdU labelling increased during low tide and decreased during high tide. Clearcut mitotic figures were identified in digestive cells, thereby confirming that mature cell types proliferate, in agreement with results from immunohistochemistry for proliferating cell nuclear antigen and BrdU. Epithelial cell renewal in the stomach also appeared to be synchronised.This investigation was funded by the Basque Government (GVPI95-36 and GVP99-1) and by a grant to Consolidated Research Groups (UPV/EHU) |
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Keywords: | Bromodeoxyuridine Cell renewal Mitosis Photoperiod Bivalves Mytilus galloprovincialis (Mollusea) |
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