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Hypoxia-inducible factor-1alpha regulates autophagy to activate hepatic stellate cells |
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| Authors: | Jing Deng Qin Huang Yueqin Wang Pei Shen Fei Guan Jianrong Li Hanju Huang Chunwei Shi |
| Institution: | 1. Department of Pathogen Biology, School of Basic Medicine, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, PR China;2. Department of Parasitology, School of Basic Medicine, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, PR China;3. Department of Medical Rehabilitation, Union Hospital, Huazhong University of Science and Technology, Wuhan 430030, PR China |
| Abstract: | The role of autophagy in Hif-1α modulated activation of hepatic stellate cells was illustrated in current work. Autophagy markers were determined in livers of Schistosoma japonicum infected mice and hypoxia or LPS treated human hepatic stellate cell, LX-2 cells. The action of Hif-1 to autophagy was defined as increase of autophagy markers was significantly suppressed in Hif-1α siRNA transfected cells upon hypoxia or LPS stimulation. The function of autophagy in activation of LX-2 cells was assessed as increase of activation markers was blocked using autophagy inhibitors under hypoxia and LPS stimulation. Conclusively, Hif-1α regulates activation of hepatic stellate cell by modulating autophagy. |
| Keywords: | HSC hepatic stellate cell Hif-1α hypoxia-inducible factor-1α α-SMA α-smooth muscle actin LC3B microtubule-associated protein light chain3B VEGF vascular endothelial growth factor LPS lipopolysaccharide ECM extracellular matrix MFC myofibroblast-like cell ATG autophagy-related gene |
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