Specific G2 phase toxicity of chloramphenicol on mouse leukemic cells (L5178Y)

The effect of chloramphenicol on progression through the cell cycle of L5178Y cells was investigated. Using eosin staining as a viability index, G2 cells were shown to be specifically killed at a concentration of chloramphenicol generally used to study mitochondrial protein synthesis. Pretreating cells with chloramphenicol induced resistance to this G2 lethality.