Development of eighteen tetranucleotide microsatellite markers in Tibetan Macaque (Macaca thibetana) and genetic diversity analysis of captive population |
| |
| Institution: | 1. Universidade Federal do Pampa, Curso de Medicina Veterinária, Uruguaiana, RS, Brazil;2. Universidade de São Paulo, Faculdade de Medicina Veterinária e Zootecnia, Departamento de Reprodução Animal, Pirassununga, SP, Brazil;3. Universidade de São Paulo, Escola Superior de Agricultura “Luiz de Queiroz”, Departamento de Zootecnia, Piracicaba, SP, Brazil;4. Universidade de São Paulo, Faculdade de Zootecnia e Engenharia de Alimentos, Pirassununga, SP, Brazil;5. Universidade Estadual Paulista “Júlio de Mesquita Filho”, Campus Experimental de Dracena, Dracena, SP, Brazil;6. University of Antwerp, Faculty of Pharmaceutical, Biomedical and Veterinary Sciences, Wilrijk, Belgium;1. Department of Biology, University of Rome Tor Vergata, Via Della Ricerca Scientifica, Rome 00133, Italy;2. Institute of Translational Pharmacology, National Research Council, CNR, Via Del Fosso del Cavaliere 100, 00133 Rome, Italy |
| |
| Abstract: | In this study, eighteen tetranucleotide microsatellite loci were isolated from AAAG-enriched and GATA-enriched libraries of the Tibetan macaque, Macaca thibetana. These loci were tested on 53 individuals of M. thibetana, and most loci were proved to be highly polymorphic. A total of 109 alleles were detected with an average of 6.06 alleles per locus. The PIC values of these loci ranged from 0.192 to 0.879, with an average of 0.624. The observed and expected heterozygosities ranged from 0.170 to 0.800 and from 0.217 to 0.898, with an average of 0.583 and 0.675, respectively. 5 loci significantly deviated from Hardy–Weinberg equilibrium (HWE). Significant linkage disequilibrium (LD) was found between 9 pairs of loci. The newly identified polymorphic markers would facilitate the study of M. thibetana on the population structure and genetic diversity. |
| |
| Keywords: | Enriched library Microsatellite Polymorphism |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
