Isolation and detection of differential genes in hot pepper (Capsicum annuum L.) after space flight using AFLP markers |
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| Affiliation: | 1. Horticultural Sub-Academy, Heilongjiang Academy of Agricultural Sciences, Harbin, Heilongjiang 150069, China;2. College of Life Science, Northeast Forestry University, Harbin, Heilongjiang 150040, China;3. Institute of Crop Science, Chinese Academy of Agricultural Sciences/National Center of Space Mutagenesis for Crop Improvement, Beijing 100081, China;1. Department of Chemical and Materials Engineering, Hefei University, 99 Jinxiu Road, Hefei 230601, PR. China;2. Department of Mechanical Engineering, Hefei University, 99 Jinxiu Road, Hefei 230601, PR. China;3. Institute of Tribology, Hefei University of Technology, 193 Tunxi Road, Hefei 230009, PR. China;4. Mechanical Engineering, School of Engineering, University of Birmingham, Edgbaston, Birmingham, B15 2TT, United Kingdom;1. Department of Nuclear Medicine, Yonsei University College of Medicine, Gangnam Severance Hospital, 712, Eonjuro, Gangnam-Gu, Seoul 135-720, Republic of Korea;2. Department of Anatomy, Yonsei University Wonju College of Medicine, Wonju 220-701, Republic of Korea;3. Department of Neurology, Yonsei University College of Medicine, Gangnam Severance Hospital, Seoul 135-720, Republic of Korea;4. Department of Pharmacology, Brain Research Institute, Brain Korea 21 Project for Medical Science, Yonsei University College of Medicine, Seoul 120-752, Republic of Korea;5. Department of Chemistry and Biochemistry, Swarthmore College, 500 College Avenue Swarthmore, PA 19081, USA;6. Department of Molecular Imaging, Korea Institute of Radiological & Medical Sciences, Seoul 139-706, Republic of Korea;1. Universidade Estadual de Maringá, Departamento de Química, Av. Colombo 5790, 87020-900, Maringá, PR, Brazil;2. Universidade Estadual de Ponta Grossa, Departamento de Biologia, Campus Uvaranas, Av. General Carlos Cavalcanti, 4748, 84030-900, Ponta Grossa, PR, Brazil;1. Fish Genetics and Biotechnology Division, Central Institute of Freshwater Aquaculture, Kausalyaganga, Bhubaneswar 751002, Odisha, India;2. Anand Agricultural University, Anand 388110, Gujarat, India |
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| Abstract: | Hot pepper (Capsicum annuum L.) is used as an important crop all over the world. To detect the differential genes of hot pepper mutants, three space-induced mutants (Y1, Y2 and Y3), which obtained new traits after space flight compared with their on-ground controls (W1 and W2), were firstly analyzed using amplified fragment length polymorphisms (AFLPs) markers. In this study, 37 out of random-selected 256 primer combinations could amplify clear high-intensity, reproducible and relatively high polymorphism bands. Accordingly, 287 different bands based on presence/absence or differences in intensity were identified and sequenced, 68 differential genes were classified functionally into different groups using Gene Ontology (GO) terms. According to GO annotation, 68 expressed sequence tags (ESTs) were homologous with known genes, which mainly included transcription factors, protein kinases, metabolic pathways, cellular processes and stress injury. In conclusion, these findings could provide the fundamental basis for further research in breeding hot pepper with high yield or the mechanism of space inducement. |
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| Keywords: | Space-inducement AFLP |
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