Isolation and characterization of thirteen polymorphic microsatellite loci for Lithocarpus glaber |
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Institution: | 1. Department of Biomechatronics Engineering, National Taiwan University, Taipei, Taiwan;2. The Experimental Forest, College of Bio-Resources and Agriculture, National Taiwan University, Nantou, Taiwan;3. Department of Forestry, National Pingtung University of Science and Technology, Pingtung, Taiwan;4. School of Forestry and Resource Conservation, National Taiwan University, Taipei, Taiwan |
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Abstract: | We isolated and characterized polymorphic microsatellite loci in Lithocarpus glaber (Fagaceae), an evergreen broadleaved monoecious tree, to provide tool for analyzing genetic structure and diversity. Thirteen polymorphic microsatellite loci were developed and tested in two L. glaber populations. The number of alleles per locus varied from 2 to 19. The observed and expected heterozygosities within populations were 0.037–0.833 and 0.316–0.931, respectively. Four loci significantly deviated from Hardy–Weinberg equilibrium after Bonferroni correction in each population and no significant linkage disequilibrium between pairs of loci was found. These polymorphic loci showed high levels of polymorphism within tested populations and will be useful in further population genetic studies. |
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Keywords: | Microsatellites primers Evergreen broadleaved forest Fagaceae Subtropical China |
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