Discrimination and genetic diversity of cultivated and wild safflowers (Carthamus spp.) using EST-microsatellites markers |
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| Institution: | 1. Department of Earth Sciences, Centre for Earth Resources Research, Memorial University of Newfoundland, St. John''s, Newfoundland and Labrador A1B 3X5, Canada;2. Department of Geological Engineering, Faculty of Mines, Istanbul Technical University, Ayazağa, Istanbul 34426, Turkey;3. Institute of Marine Sciences and Technology, Dokuz Eylül University, Haydar Aliyev Caddesi No: 10, İnciraltı, İzmir 35340, Turkey;1. Dipartimento di Scienze, Università degli Studi Roma Tre, Largo S. Leonardo Murialdo, 1, I-00146 Roma, Italy;2. Geological Consultant, Vasto, CH, Italy;3. Informal Group of Micropaleontological and Malacological Researches, Italy;4. Dipartimento di Scienze della Terra, Università degli Studi “La Sapienza” di Roma, Piazzale A. Moro, 5, I-00185 Roma, Italy;5. Saint-Gobain PPC Italia S.p.A., via Ettore Romagnoli 6, 20146 Milano, Italy;6. CNR - Dipartimento Scienze del Sistema Terra e Tecnologie per l''Ambiente, Piazzale Aldo Moro, 7, I-00185 Roma, Italy;7. Institut des Sciences de l''Évolution, UMR 5554/CNRS—Palaeoenvironments, Université Montpellier 2, C.P. 061, Place E. Bataillon, F34095 Montpellier, Cedex 5, France;1. Departamento de Geodinámica, Facultad de Ciencias, Universidad de Granada, Fuentenueva s/n, 18071 Granada, Spain;2. Instituto Andaluz Ciencias de la Tierra, Consejo Superior de Investigación Científica, Avenida de las Palmeras 4, 18100 Armilla, Granada, Spain;3. Departamento de Sistemas Físicos, Químicos y Naturales, Universidad Pablo de Olavide, Ctra. de Utrera km. 1, 41013 Sevilla, Spain;1. Department of Biochemistry and Molecular Biology, Virginia Commonwealth University, Richmond, VA 23298, USA;2. Sanger Building, Room 2-032b, 1101 East Marshall Street, Richmond, VA 23298, USA |
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| Abstract: | In spite of being one of the major oilseed crops, little is known about genetic diversity and relationships between species of safflower. In this study EST-SSR markers were used to evaluate and characterize 42 genotypes from six species including Carthamus tinctorius, Carthamus palaestinus, Carthamus oxyacanthus, Carthamus lanatus, Carthamus dentatus, and Carthamus boissieri. Thirty three primer pairs produced 123 polymorphic bands with 2–8 alleles per locus. The EST-SSR markers showed different level of gene diversity. The highest Polymorphism Information Content (PIC) values were observed for primers EL510507 and EL390720 (0.49 and 0.45, respectively). The highest genetic diversity and heterozygosity were observed for C. oxyacanthus. Both cluster and principal coordinate analysis (PCoA) clearly separated species into distinct groups. Within each species the accessions were clustered in different subgroups that mainly supported the known origins. The result showed that C. palaestinus had the most genetic similarity with cultivated safflower and C. oxyacanthus was next in this respect. In general, EST-SSR markers effectively revealed the genetic relationships and diversity of Carthamus species. This information is valuable for safflower improvement since C. palaestinus and C. oxyacanthus are both crossable with the cultivated species C. tinctorius. |
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| Keywords: | EST-SSR Safflower Wild relatives Genetic diversity |
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