Reconstitution of the GalP galactose transport activity of Escherichia coli into liposomes made from soybean phospholipids |
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| Authors: | Peter J.F. Henderson Yasuo Kagawa Hajime Hirata |
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| Affiliation: | Department of Biochemistry, Jichi Medical School, Minamikawachi-machi, Kawachi-gun, Tochigi-ken, 329-04 Japan |
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| Abstract: | Galactose transport activity from Escherichia coli was solubilized with octyl glucoside, and reconstituted into liposomes made from soybean or E. coli lipid. Galactose counterflow in the proteoliposomes was inhibited by glucose, talose, 2-deoxygalactose and 6-deoxygalactose, confirming that it was due to GalP and not one of the other E. coli galactose transport systems. |
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| Keywords: | Galactose transport Membrane reconstitution Liposome (E. coli) GalP and MglP To whom correspondence should be sent at (permanent address): Department of Biochemistry, University of Cambridge, Tennis Court Road, Cambridge CB2 1QW, U.K. |
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