Effect of poly(ethylene glycol) on phospholipid hydration and polarity of the external phase |
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Authors: | Klaus Arnold Lothar Pratsch Klaus Gawrisch |
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Institution: | 1. Sektion Physik der Karl-Marx-Universität, Bereich Molekülphysik, Linnestr. 5, 7010 Leipzig G.D.R.;2. Sektion Biologie der Humboldt-Universität, Bereich Biophysik, Invalidenstr. 42, 1040 Berlin G.D.R. |
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Abstract: | The hydration properties of phosphatidylcholine (PC)/water dispersions on the addition of poly(ethylene glycol) were studied by means of 2H-NMR. The quadrupole splittings and their temperature dependences correspond to measurements of PC/water dispersions at low water content. It is concluded that the bound water is partly extracted by poly(ethylene glycol) but the binding properties of the water in the inner hydration shell of about five water molecules are not changed. The ability of some phospholipid/water dispersions to undergo phase transitions to nonlamellar structures upon dehydration is discussed. Dipalmitoylphosphatidylcholine (DPPC) and egg phosphatidylcholine do not form nonlamellar structures on addition of purified poly(ethylene glycol), as was demonstrated by means of 31P-NMR. Poly(ethylene glycol) decreases the polarity of the aqueous phase and the partition of hydrophobic molecules between the membrane and the external phase is changed. This was demonstrated using the excimer fluorescence of pyrene in a ghost suspension. It is suggested that the changes in polarity and hydration on the addition of poly(ethylene glycol) can contribute to the alterations in the membrane surface observed under conditions of membrane contact and fusion. |
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Keywords: | Membrane fusion Poly(ethylene glycol) Hydration Phospholipid Fluorescence ANS 1-anilinonaphthalene-8-sulfonate DPPC PC phosphatidylcholine |
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