An X-ray diffraction study on phase transition temperatures of various membranes isolated from Tetrahymena pyriformis cells grown at different temperatures |
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Authors: | Haruto Nakayama Masako Goto Kazuo Ohki Toshio Mitsui Yoshinori Nozawa |
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Institution: | 1. Department of Biophysical Engineering, Faculty of Engineering Science. Osaka University, Toyonaka, Osaka 560 Japan;2. Department of Biochemistry, Gifu University School of Medicine, Tsukasamachi-40, Gifu 500 Japan |
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Abstract: | Mitochondrial, microsomal and pellicular membranes were isolated from Tetrahymena cells grown at 39°C or 15°C, and phospholipids, in turn, were separated from total lipids extracted from these membranes. The effect of growth temperature on their solid-to-fluid phase transition temperature was examined by wide-angle X-ray diffraction. The transition temperatures of phospholipids from mitochondria, microsomes and pellicles were 21, 19 and 26°C for cells grown at 39°C and ?8, ?3 and 6°C for cells grown at 15°C, respectively. All phospholipids were found in a completely fluid state at these growth temperatures. From a comparison between the phospholipids and total lipids from pellicles of cells grown at 39°C, a triterpenoid alcohol, tetrahymanol, caused the transition temperature to increase. The alignment of tetrahymanol in membranes was examined with pellicle's total lipid oriented in a sample holder. |
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Keywords: | Temperature acclimation Membrane fluidity Phase transition X-ray diffraction Tetrahymanol (Tetrahymena) |
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