The application of a potential-sensitive cyanine dye to rat small intestinal brush border membrane vesicles |
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| Authors: | B Stieger G Burckhardt H Murer |
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| Institution: | 1. Department of Physiology, University of Zurich, Rämistrasse 69, CH-8028 Zürich Switzerland;2. Max-Planck-Institut für Biophysik, Kennedy-allee 70, D-6 Frankfurt/M 70 F.R.G. |
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| Abstract: | The sensitivity of the fluorescent dye, 3,3′-diethylthiadicarbocyanine (DiS-C2(5)), was too low for the detection of membrane potential changes in rat small intestinal membrane vesicles. Only after adding LaCl3 or after fractionation of the intestinal membranes by free-flow electrophoresis could the dye be used to monitor electrogenic Na+-dependent transport systems. It is concluded that the response of this potential-sensitive dye is influenced by the negative surface charge density of the vesicles. |
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| Keywords: | Membrane potential Fluorescence Cyanine dye (Rat intestine) Hepes 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid 3 3′-diethylthiadicarbocyanine iodide |
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