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A structural investigation of cytochrome c binding to photosynthetic reaction centers in reconstituted membranes |
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| Authors: | James M Pachence PLeslie Dutton JKent Blasie |
| Institution: | 1. Department of Chemistry, University of Pennsylvania, Philadelphia, PA 1904 U.S.A.;2. Department of Biochemistry/Biophysics, University of Pennsylvania, Philadelphia, PA 1904 U.S.A.;3. Department of Biology, Brookhaven National Laboratory, Upton, NY 11974 U.S.A.;4. Department of Biochemistry, Columbia University, College of Physicians and Surgeons, New York, NY 10032 U.S.A. |
| Abstract: | Mammalian cytochrome c can effectively replace bacterial cytochrome c2 as the electron donor to the bacterial photosynthetic reaction center in either the natural chromatophore or a reconstituted reaction center/phospholipid membrane. In this paper, the reconstituted membrane was used to describe the nature of cytochrome c binding to the reaction center, the location of bound cytochrome c in the membrane profile and the perturbation of the reaction center and phospholipid profile structures induced by cytochrome c binding. These structural studies utilized the combined techniques of X-ray and neutron diffraction. |
| Keywords: | Photosynthesis Reaction center Membrane reconstitution X-ray diffraction Neutron diffraction |
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