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Fusion of mitochondrial inner membranes by electric fields produces inside-out vesicles: Visualization by freeze-fracture electron microscopy |
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| Authors: | Arthur E. Sowers |
| Affiliation: | Laboratories for Cell Biology, Department of Anatomy, University of North Carolina at Chapel Hill, Chapel Hill, NC 27514 U.S.A. |
| Abstract: | The fusion of vesicular-shaped mitochondrial inner membranes was observed by a new approach which combines freeze-fracture electron microscopy and electric field-induced fusion. Results show that membrane events caused by the exposure to the electric field can be time-coordinated with sample freezing for subsequent analysis by freeze-fracture electron microscopy. |
| Keywords: | Membrane fusion Electric field Electron microscopy Freeze-fracture Mitochondrial membrane diffusion coefficient half distance between electrodes M mitochondrial inner membrane spheres MFP main fusion product R.M.S. root-mean-square S.D. standard deviation SFP small vesicular membrane fusion product time |
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