Nutritional assessment and fate of dna of soybean meal from roundup ready or conventional soybeans using rats |
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Authors: | Yuanzhao Zhu Fenglai Wang Jingdong Yin Hong Jin |
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Affiliation: | 1. National Feed Engineering Technology Research Center , China Agricultural University , Beijing , China;2. Animal Veterinary College , China Agricultural University , Beijing , China |
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Abstract: | This study was conducted to compare the safety of soybean meal prepared from genetically modified (GM) glyphosate-tolerant (Roundup Ready; RR) soybeans and conventional soybeans. Eighty Sprague-Dawley rats (40 males and 40 females) were randomly allotted to one of four groups according to sex and body weight for a 13-week feeding experiment. The rats were fed corn-based diets containing 60% conventional soybean meal, a mixture of 30% conventional and 30% RR soybean meal, 60% or 90% RR soybean meal. All diets were adjusted to an identical nutrient level except the 90% RR diet. The two soybean meals were similar in chemical analysis and amino acid composition. During the 13-week growth trial, body weight (P?0.05) and feed intake (P?0.05) decreased only in rats fed with 90% RR soybean meal at the first week. No treatment-related deaths occurred during the experiment. Gross necropsy findings, haematological or urinalysis values and clinical serum parameters showed no meaningful differences between rats fed the control and RR soybean meals. A 145?bp of cp4 epsps gene specific for the GM constructs from RR soybean meal or a 407?bp of lec gene from endogenous soybean DNA could not be detected in investigated masseter muscle samples. No adverse effects of glyphosate-tolerant soybean meal on rats were seen even at levels as high as 90% of the diet. |
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Keywords: | GM soybean meal Safety assessment Rats PCR analysis Subchronic feeding |
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