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Ethanol production by <Emphasis Type="Italic">Saccharomyces cerevisiae</Emphasis> and <Emphasis Type="Italic">Kluyveromyces marxianus</Emphasis> in the presence of orange-peel oil
Authors:Mark R Wilkins  Lilis Suryawati  Niels O Maness  Donna Chrz
Institution:(1) Department of Biosystems and Agricultural Engineering, Oklahoma State University, 111 Agricultural Hall, Stillwater, OK 74078, USA;(2) Department of Horticulture and Landscape Architecture, Oklahoma State University, 360 Agricultural Hall, Stillwater, OK 74078, USA
Abstract:Summary Two ethanologenic yeasts, Saccharomyces cerevisiae and Kluyveromyces marxianus, were used to ferment sugar solutions modeling hydrolyzed Valencia orange peel waste at 37°C. Orange stripper oil produced from orange peel was added in various amounts to determine its effect on ethanol production. The minimum peel oil concentration that inhibited ethanol production was determined after 24, 48 and 72 h and the two yeasts were compared to one another in terms of ethanol yield. Minimum inhibitory peel oil concentrations for ethanol production were 0.05% at 24 h, 0.10% at 48 h, and 0.15% at 72 h for both yeasts. S. cerevisiae produced more ethanol than K. marxianus at each time point.
Keywords:Bioprocessing  Citrus  Ethanol  Fermentation  Limonene  Yeast
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