Isolation of sperm cells from mature pollen grains of Spinacia oleracea L. |
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| Authors: | C. H. Theunis J. L. Van Went |
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| Affiliation: | (1) Department of Plant Cytology and Morphology, Agricultural University of Wageningen, Arboretumlaan 4, NL-6703 BD Wageningen, The Netherlands |
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| Abstract: | Summary When mature pollen grains of Spinacia oleracea were squashed in a 25% sucrose solution and subsequently centrifuged on a percoll layer, sperm cells were isolated in high numbers. All steps were carried out at 4° C. Isolated sperm cells could be kept alive for several hours. |
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| Keywords: | Sperm cell isolation Pollen Spinacia oleracea |
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